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Double Helix double helix psf dh psf microscopy
Detection <t>PSF</t> for <t>a</t> <t>Double-Helix</t> (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.
Double Helix Psf Dh Psf Microscopy, supplied by Double Helix, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Universal approach to wave-optical calculations of point spread functions in microscopy (and beyond)"

Article Title: Universal approach to wave-optical calculations of point spread functions in microscopy (and beyond)

Journal: bioRxiv

doi: 10.64898/2026.04.28.721333

Detection PSF for a Double-Helix (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.
Figure Legend Snippet: Detection PSF for a Double-Helix (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.

Techniques Used:

PSF of SOFI up to the fourth order. a = wide-field PSF, b = 2 nd order SOFI, c = 3 rd order SOFI, d = 4 th order SOFI. Please note the reduction of PSF size along all three dimensions. Already 2 nd order SOFI provides optical sectioning as in CLSM or two-photon excitation scanning microscopy, in contrast to the wide-field microscope (panel a) used for recording the images from which SOFI is calculated.
Figure Legend Snippet: PSF of SOFI up to the fourth order. a = wide-field PSF, b = 2 nd order SOFI, c = 3 rd order SOFI, d = 4 th order SOFI. Please note the reduction of PSF size along all three dimensions. Already 2 nd order SOFI provides optical sectioning as in CLSM or two-photon excitation scanning microscopy, in contrast to the wide-field microscope (panel a) used for recording the images from which SOFI is calculated.

Techniques Used: Microscopy

PSF of widefield iSCAT microscopy. Panel a: Schematic of wide-field iSCAT microscopy. Panels b-e: The top row of the figure displays the z-scan PSF of wide-field iSCAT microscopy under optically perfect conditions, where the refractive index of the 170 µm thick coverslip matches the refractive index of the objective’s immersion medium ( n glass = 1.51). In contrast, the bottom row illustrates the resulting z -scan PSF when the refractive index of the coverslip deviates by only 0.005 from the ideal value ( n glass = 1.505), a minor mismatch that nonetheless induces perceptible changes in the signal distribution. For both cases, the left panels show the xz -cross-sections of the PSF, while the right panels display the xy -cross-sections taken at the plane of maximum absolute signal.
Figure Legend Snippet: PSF of widefield iSCAT microscopy. Panel a: Schematic of wide-field iSCAT microscopy. Panels b-e: The top row of the figure displays the z-scan PSF of wide-field iSCAT microscopy under optically perfect conditions, where the refractive index of the 170 µm thick coverslip matches the refractive index of the objective’s immersion medium ( n glass = 1.51). In contrast, the bottom row illustrates the resulting z -scan PSF when the refractive index of the coverslip deviates by only 0.005 from the ideal value ( n glass = 1.505), a minor mismatch that nonetheless induces perceptible changes in the signal distribution. For both cases, the left panels show the xz -cross-sections of the PSF, while the right panels display the xy -cross-sections taken at the plane of maximum absolute signal.

Techniques Used: Microscopy, Refractive Index

Related Articles

Isolation:

Article Title: 3D Super-Resolution Imaging of PSD95 Reveals an Abundance of Diffuse Protein Supercomplexes in the Mouse Brain.
Article Snippet: PSF engineering methods include astigmatism (<1 μm DoF),30 the double-helix PSF (DHPSF, 2−4 μm DoF),31 the tetrapod PSF (4−8 μm DoF),32,33 and single-molecule light field microscopy (∼8 μm DoF).34,35 The DHPSF is well-suited to the investigation of sectioned tissue by affording greater resolution isotropy (xy: ∼ 10 nm and z: ∼ 20 nm)36 compared to astigmatism37 and a large ∼4 μm DoF that eliminates the need for axial scanning.38 We present the first application of DHPSF SR microscopy in tissue, enabling the most comprehensive study of the 3D organization of individual PSD95 molecules.

Article Title: One-click image reconstruction in single-molecule localization microscopy via deep learning
Article Snippet: Next, we evaluated a dataset featuring a double-helix PSF, consisting of 80k frames (272 by 198 pixels, video 2).

Article Title: 3D target for optical system characterization
Article Snippet: The engineered PSF can be a double-helix PSF or a tetrapod PSF.

Article Title: 3D Super-Resolution Imaging of PSD95 Reveals an Abundance of Diffuse Protein Supercomplexes in the Mouse Brain
Article Snippet: PSF engineering methods include astigmatism (<1 μm DoF), the double-helix PSF (DHPSF, 2–4 μm DoF), the tetrapod PSF (4–8 μm DoF), , and single-molecule light field microscopy (∼8 μm DoF)., The DHPSF is well-suited to the investigation of sectioned tissue by affording greater resolution isotropy ( xy : ∼ 10 nm and z : ∼ 20 nm) compared to astigmatism and a large ∼4 μm DoF that eliminates the need for axial scanning.

Article Title: A relay race of ESCRT-III paralogs drives cell division in a hyperthermophilic archaeon.
Article Snippet: The double-helix PSF was chosen because its depth of imaging (~3 μm) allows to image rings with large diameters (>1 μm), and at different depths.

Article Title: Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet
Article Snippet: The authors have also added repeated measurements demonstrating the improved localization precision due to background reduction when using the light sheet versus epi.In their rebuttal letter, the authors compare the soTILT3D resolution of 8 nm lateral and 12 nm axial to Pavani et al. (PNAS 2009), who achieved 10 nm lateral and 20 nm axial using the double-helix PSF.

Article Title: Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet
Article Snippet: In their rebuttal letter, the authors compare the soTILT3D resolution of 8 nm lateral and 12 nm axial to Pavani et al. (PNAS 2009), who achieved 10 nm lateral and 20 nm axial using the double-helix PSF.

Article Title: Optimized molecule detection in localization microscopy with selected false positive probability
Article Snippet: Effectively, I believe this manuscript would benefit enormously from including more exotic PSF shapes, for instance the Double-Helix PSF used in the SMLM challenge (2013).

Imaging:

Article Title: 3D Super-Resolution Imaging of PSD95 Reveals an Abundance of Diffuse Protein Supercomplexes in the Mouse Brain.
Article Snippet: PSF engineering methods include astigmatism (<1 μm DoF),30 the double-helix PSF (DHPSF, 2−4 μm DoF),31 the tetrapod PSF (4−8 μm DoF),32,33 and single-molecule light field microscopy (∼8 μm DoF).34,35 The DHPSF is well-suited to the investigation of sectioned tissue by affording greater resolution isotropy (xy: ∼ 10 nm and z: ∼ 20 nm)36 compared to astigmatism37 and a large ∼4 μm DoF that eliminates the need for axial scanning.38 We present the first application of DHPSF SR microscopy in tissue, enabling the most comprehensive study of the 3D organization of individual PSD95 molecules.

Article Title: One-click image reconstruction in single-molecule localization microscopy via deep learning
Article Snippet: Next, we evaluated a dataset featuring a double-helix PSF, consisting of 80k frames (272 by 198 pixels, video 2).

Article Title: 3D target for optical system characterization
Article Snippet: The engineered PSF can be a double-helix PSF or a tetrapod PSF.

Article Title: 3D Super-Resolution Imaging of PSD95 Reveals an Abundance of Diffuse Protein Supercomplexes in the Mouse Brain
Article Snippet: PSF engineering methods include astigmatism (<1 μm DoF), the double-helix PSF (DHPSF, 2–4 μm DoF), the tetrapod PSF (4–8 μm DoF), , and single-molecule light field microscopy (∼8 μm DoF)., The DHPSF is well-suited to the investigation of sectioned tissue by affording greater resolution isotropy ( xy : ∼ 10 nm and z : ∼ 20 nm) compared to astigmatism and a large ∼4 μm DoF that eliminates the need for axial scanning.

Article Title: A relay race of ESCRT-III paralogs drives cell division in a hyperthermophilic archaeon.
Article Snippet: The double-helix PSF was chosen because its depth of imaging (~3 μm) allows to image rings with large diameters (>1 μm), and at different depths.

Article Title: Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet
Article Snippet: The authors have also added repeated measurements demonstrating the improved localization precision due to background reduction when using the light sheet versus epi.In their rebuttal letter, the authors compare the soTILT3D resolution of 8 nm lateral and 12 nm axial to Pavani et al. (PNAS 2009), who achieved 10 nm lateral and 20 nm axial using the double-helix PSF.

Article Title: Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet
Article Snippet: In their rebuttal letter, the authors compare the soTILT3D resolution of 8 nm lateral and 12 nm axial to Pavani et al. (PNAS 2009), who achieved 10 nm lateral and 20 nm axial using the double-helix PSF.

Article Title: Optimized molecule detection in localization microscopy with selected false positive probability
Article Snippet: Effectively, I believe this manuscript would benefit enormously from including more exotic PSF shapes, for instance the Double-Helix PSF used in the SMLM challenge (2013).



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Detection <t>PSF</t> for <t>a</t> <t>Double-Helix</t> (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.
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Detection <t>PSF</t> for <t>a</t> <t>Double-Helix</t> (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.
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Detection <t>PSF</t> for <t>a</t> <t>Double-Helix</t> (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.
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Detection <t>PSF</t> for <t>a</t> <t>Double-Helix</t> (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.
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Detection <t>PSF</t> for <t>a</t> <t>Double-Helix</t> (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.
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Detection <t>PSF</t> for <t>a</t> <t>Double-Helix</t> (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.
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Detection <t>PSF</t> for <t>a</t> <t>Double-Helix</t> (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.
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Image Search Results


Detection PSF for a Double-Helix (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.

Journal: bioRxiv

Article Title: Universal approach to wave-optical calculations of point spread functions in microscopy (and beyond)

doi: 10.64898/2026.04.28.721333

Figure Lengend Snippet: Detection PSF for a Double-Helix (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.

Article Snippet: Double-Helix PSF (DH-PSF) microscopy is a powerful wide-field technique designed to extend the axial tracking range and precision of single-molecule localization microscopy.

Techniques:

PSF of SOFI up to the fourth order. a = wide-field PSF, b = 2 nd order SOFI, c = 3 rd order SOFI, d = 4 th order SOFI. Please note the reduction of PSF size along all three dimensions. Already 2 nd order SOFI provides optical sectioning as in CLSM or two-photon excitation scanning microscopy, in contrast to the wide-field microscope (panel a) used for recording the images from which SOFI is calculated.

Journal: bioRxiv

Article Title: Universal approach to wave-optical calculations of point spread functions in microscopy (and beyond)

doi: 10.64898/2026.04.28.721333

Figure Lengend Snippet: PSF of SOFI up to the fourth order. a = wide-field PSF, b = 2 nd order SOFI, c = 3 rd order SOFI, d = 4 th order SOFI. Please note the reduction of PSF size along all three dimensions. Already 2 nd order SOFI provides optical sectioning as in CLSM or two-photon excitation scanning microscopy, in contrast to the wide-field microscope (panel a) used for recording the images from which SOFI is calculated.

Article Snippet: Double-Helix PSF (DH-PSF) microscopy is a powerful wide-field technique designed to extend the axial tracking range and precision of single-molecule localization microscopy.

Techniques: Microscopy

PSF of widefield iSCAT microscopy. Panel a: Schematic of wide-field iSCAT microscopy. Panels b-e: The top row of the figure displays the z-scan PSF of wide-field iSCAT microscopy under optically perfect conditions, where the refractive index of the 170 µm thick coverslip matches the refractive index of the objective’s immersion medium ( n glass = 1.51). In contrast, the bottom row illustrates the resulting z -scan PSF when the refractive index of the coverslip deviates by only 0.005 from the ideal value ( n glass = 1.505), a minor mismatch that nonetheless induces perceptible changes in the signal distribution. For both cases, the left panels show the xz -cross-sections of the PSF, while the right panels display the xy -cross-sections taken at the plane of maximum absolute signal.

Journal: bioRxiv

Article Title: Universal approach to wave-optical calculations of point spread functions in microscopy (and beyond)

doi: 10.64898/2026.04.28.721333

Figure Lengend Snippet: PSF of widefield iSCAT microscopy. Panel a: Schematic of wide-field iSCAT microscopy. Panels b-e: The top row of the figure displays the z-scan PSF of wide-field iSCAT microscopy under optically perfect conditions, where the refractive index of the 170 µm thick coverslip matches the refractive index of the objective’s immersion medium ( n glass = 1.51). In contrast, the bottom row illustrates the resulting z -scan PSF when the refractive index of the coverslip deviates by only 0.005 from the ideal value ( n glass = 1.505), a minor mismatch that nonetheless induces perceptible changes in the signal distribution. For both cases, the left panels show the xz -cross-sections of the PSF, while the right panels display the xy -cross-sections taken at the plane of maximum absolute signal.

Article Snippet: Double-Helix PSF (DH-PSF) microscopy is a powerful wide-field technique designed to extend the axial tracking range and precision of single-molecule localization microscopy.

Techniques: Microscopy, Refractive Index