double helix psf dh psf microscopy (Double Helix)
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Double Helix Psf Dh Psf Microscopy, supplied by Double Helix, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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1) Product Images from "Universal approach to wave-optical calculations of point spread functions in microscopy (and beyond)"
Article Title: Universal approach to wave-optical calculations of point spread functions in microscopy (and beyond)
Journal: bioRxiv
doi: 10.64898/2026.04.28.721333
Figure Legend Snippet: Detection PSF for a Double-Helix (DH) waveplate. Panel a: Displays the imposed phase modulation applied to the incoming wavefront in the BFP, following the design proposed in Ref. . Panel b: Presents the resulting detection PSF as a 3D visualization, where nine distinct intensity isosurfaces are plotted to enhance the visibility of the intricate helical intensity structure. Panel c: Shows five lateral ( xy ) cross-sections of the PSF at the indicated z -values, illustrating the rotation of the lobes as a function of axial position.
Techniques Used:
Figure Legend Snippet: PSF of SOFI up to the fourth order. a = wide-field PSF, b = 2 nd order SOFI, c = 3 rd order SOFI, d = 4 th order SOFI. Please note the reduction of PSF size along all three dimensions. Already 2 nd order SOFI provides optical sectioning as in CLSM or two-photon excitation scanning microscopy, in contrast to the wide-field microscope (panel a) used for recording the images from which SOFI is calculated.
Techniques Used: Microscopy
Figure Legend Snippet: PSF of widefield iSCAT microscopy. Panel a: Schematic of wide-field iSCAT microscopy. Panels b-e: The top row of the figure displays the z-scan PSF of wide-field iSCAT microscopy under optically perfect conditions, where the refractive index of the 170 µm thick coverslip matches the refractive index of the objective’s immersion medium ( n glass = 1.51). In contrast, the bottom row illustrates the resulting z -scan PSF when the refractive index of the coverslip deviates by only 0.005 from the ideal value ( n glass = 1.505), a minor mismatch that nonetheless induces perceptible changes in the signal distribution. For both cases, the left panels show the xz -cross-sections of the PSF, while the right panels display the xy -cross-sections taken at the plane of maximum absolute signal.
Techniques Used: Microscopy, Refractive Index
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